GREEN PROCEDURE INDEX ASSESSMENT OF THE NOVEL STABILITY-INDICATING RP-HPLC METHOD FOR THE DETERMINATION OF CAPTOPRIL FROM PHARMACEUTICAL DOSAGE FORM

Objective: In this study, it was aimed to develop a novel reverse-phase liquid chromatography method for the ultra-sensitive determination of antihypertensive drug captopril, using paracetamol, which is common pain killer, as internal standard. Optimization all experimental conditions including composition mobile phase, flow rate, and column temperature carried out step by step, validity developed examined according international validation guidelines. Calibration range, linearity, limit determination, quantification, robustness, accuracy from commercial tablet samples, stability were in detail. addition, greenness profile assessed with Green Analytical Procedure Index Greenness Calculator techniques, are frequently used literature. Material Method: The chromatographic conducted an XBridge C18 (25 cm x 4.6 mm ID; 5 µm) packed fully porous silica materials. All analyses performed isocratically phase containing acetonitrile:5 mM, pH 7.0 ammonium acetate solution (50:50, v/v) at rate 1.5 ml min-1. injection volume μl, kept 25°C oven. eluate monitored 220 nm. Under optimized conditions, retention times paracetamol approximately 1.59, 2.0 min, respectively. Result Discussion: This study described validated, simple, sensitive, accurate, linear, precise, reproducible reversed-phase captopril samples. optimal linear range found 0.5-200 µg ml-1 correlation coefficient greater than 0.99. Method precision acceptable, coefficients variation between 0.05% 0.61%. result recovery studies on be within satisfactory limits 99.45% 102.55%. Moreover, also showed that environmentally friendly.